MicroRNAs Regulate Metabolic Phenotypes During Multicellular Tumor Spheroids Progression.

ABSTRACT: During tumor progression, cancer cells rewire their metabolism to face their bioenergetic demands. In recent years, microRNAs (miRNAs) have emerged as regulatory elements that inhibit the translation and stability of crucial mRNAs, some of them causing direct metabolic alterations in cancer. In this study, we investigated the relationship between miRNAs and their targets mRNAs that control metabolism, and how this fine-tuned regulation is diversified depending on the tumor stage. To do so, we implemented a paired analysis of RNA-seq and small RNA-seq in a breast cancer cell line (MCF7). The cell line was cultured in multicellular tumor spheroid (MCTS) and monoculture conditions. For MCTS, we selected two-time points during their development to recapitulate a proliferative and quiescent sta

SUBMITTER: Mucino-Olmos EA 

PROVIDER: S-EPMC7793838 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature