Investigating the influence of physiologically relevant hydrostatic pressure on CHO cell batch culture.

ABSTRACT: Chinese hamster ovary (CHO) cells have been the most commonly used mammalian host for large-scale commercial production of therapeutic proteins, such as monoclonal antibodies. Enhancement of productivity of these CHO cells is one of the top priorities in the biopharmaceutical industry to reduce manufacturing cost. Although there are many different methods (e.g. temperature, pH, feed) to improve protein production in CHO cells, the role of physiologically relevant hydrostatic pressure in CHO cell culture has not been reported yet. In this study, four different hydrostatic pressures (0, 30, 60, and 90 mmHg) were applied to batch CHO cells, and their cell growth/metabolism and IgG₁ production were examined. Our results indicate that hydrostatic pressure can increase the maximum cel

SUBMITTER: Shang M 

PROVIDER: S-EPMC7794228 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature