The negative elongation factor NELF promotes induced transcriptional response of Drosophila ecdysone-dependent genes.

ABSTRACT: For many years it was believed that promoter-proximal RNA-polymerase II (Pol II) pausing manages the transcription of genes in Drosophila development by controlling spatiotemporal properties of their activation and repression. But the exact proteins that cooperate to stall Pol II in promoter-proximal regions of developmental genes are still largely unknown. The current work describes the molecular mechanism employed by the Negative ELongation Factor (NELF) to control the Pol II pause at genes whose transcription is induced by 20-hydroxyecdysone (20E). According to our data, the NELF complex is recruited to the promoters and enhancers of 20E-dependent genes. Its presence at the regulatory sites of 20E-dependent genes correlates with observed interaction between the NELF-A subunit and the ec

SUBMITTER: Mazina MY 

PROVIDER: S-EPMC7794308 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature