High-resolution single-cell 3D-models of chromatin ensembles during Drosophila embryogenesis.

ABSTRACT: Single-cell chromatin studies provide insights into how chromatin structure relates to functions of individual cells. However, balancing high-resolution and genome wide-coverage remains challenging. We describe a computational method for the reconstruction of large 3D-ensembles of single-cell (sc) chromatin conformations from population Hi-C that we apply to study embryogenesis in Drosophila. With minimal assumptions of physical properties and without adjustable parameters, our method generates large ensembles of chromatin conformations via deep-sampling. Our method identifies specific interactions, which constitute 5-6% of Hi-C frequencies, but surprisingly are sufficient to drive chromatin folding, giving rise to the observed Hi-C patterns. Modeled sc-chromatins quantify chromatin hetero

SUBMITTER: Sun Q 

PROVIDER: S-EPMC7794469 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature