Extracellular vesicle-associated repetitive element DNAs as candidate osteosarcoma biomarkers.

ABSTRACT: Osteosarcoma (OS) is the most common malignant bone tumor in children and young adults. Despite that high-risk factors have been identified, no test for early detection is available. This study aimed to identify circulating nucleic acid sequences associated with serum extracellular vesicle (EV) preparations at the time of OS diagnosis, as a step towards an OS early detection assay. Sequencing of small nucleic acids extracted from serum EV preparations revealed increased representation of diverse repetitive element sequences in OS patient versus control sera. Analysis of a validation cohort using qPCR of PEG-precipitated EV preparations revealed the over-representation of HSATI, HSATII, LINE1-P1, and Charlie 3 at the DNA but not RNA level, with receiver operating characteristic (ROC) area u

SUBMITTER: Cambier L 

PROVIDER: S-EPMC7794510 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature