Copper oxide-based cathode for direct NADPH regeneration.

ABSTRACT: Nearly a fourth of all enzymatic activities is attributable to oxidoreductases, and the redox reactions supported by this vast catalytic repertoire sustain cellular metabolism. In many biological processes, reduction depends on hydride transfer from either reduced nicotinamide adenine dinucleotide (NADH) or its phosphorylated derivative (NADPH). Despite longstanding efforts to regenerate NADPH by various methods and harness it to support chemoenzymatic synthesis strategies, the lack of product purity has been a major deterrent. Here, we demonstrate that a nanostructured heterolayer Ni-Cu₂O-Cu cathode formed by a photoelectrochemical process has unexpected efficiency in direct electrochemical regeneration of NADPH from NADP⁺. Remarkably, two-thirds of NADP⁺

SUBMITTER: Kadowaki JT 

PROVIDER: S-EPMC7794519 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature