Nanoscale observation of PM2.5 incorporated into mammalian cells using scanning electron-assisted dielectric microscope.

ABSTRACT: PM2.5 has been correlated with risk factors for various diseases and infections. It promotes tissue injury by direct effects of particle components. However, effects of PM2.5 on cells have not been fully investigated. Recently, we developed a novel imaging technology, scanning electron-assisted dielectric-impedance microscopy (SE-ADM), which enables observation of various biological specimens in aqueous solution. In this study, we successfully observed PM2.5 incorporated into living mammalian cells in culture media. Our system directly revealed the process of PM2.5 aggregation in the cells at a nanometre resolution. Further, we found that the PM2.5 aggregates in the intact cells were surrounded by intracellular membrane-like structures of low-density in the SE-ADM images. Moreover, the PM2

SUBMITTER: Okada T 

PROVIDER: S-EPMC7794539 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature