Drug Screening with Genetically Encoded Fluorescent Sensors: Today and Tomorrow.

ABSTRACT: Genetically-encoded fluorescent sensors have been actively developed over the last few decades and used in live imaging and drug screening. Real-time monitoring of drug action in a specific cellular compartment, organ, or tissue type; the ability to screen at the single-cell resolution; and the elimination of false-positive results caused by low drug bioavailability that is not detected by in vitro testing methods are a few of the obvious benefits of using genetically-encoded fluorescent sensors in drug screening. In combination with high-throughput screening (HTS), some genetically-encoded fluorescent sensors may provide high reproducibility and robustness to assays. We provide a brief overview of successful, perspective, and hopeful attempts at using genetically encoded fluorescent senso

SUBMITTER: Potekhina ES 

PROVIDER: S-EPMC7794770 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature