Alterations in the Genomic Distribution of 5hmC in In Vivo Aged Human Skin Fibroblasts.

ABSTRACT: 5-Hydroxymethylcytosine (5hmC) is a functionally active epigenetic modification. We analyzed whether changes in DNA 5-hydroxymethylation are an element of age-related epigenetic drift. We tested primary fibroblast cultures originating from individuals aged 22-35 years and 74-94 years. Global quantities of methylation-related DNA modifications were estimated by the dot blot and colorimetric methods. Regions of the genome differentially hydroxymethylated with age (DHMRs) were identified by hMeDIP-seq and the MEDIPS and DiffBind algorithms. Global levels of DNA modifications were not associated with age. We identified numerous DHMRs that were enriched within introns and intergenic regions and most commonly associated with the H3K4me1 histone mark, promoter-flanking regions, and CCCTC-binding

SUBMITTER: Kolodziej-Wojnar P 

PROVIDER: S-EPMC7794952 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature