Unraveling a Force-Generating Allosteric Pathway of Actomyosin Communication Associated with ADP and P_i Release.

ABSTRACT: The actomyosin system generates mechanical work with the execution of the power stroke, an ATP-driven, two-step rotational swing of the myosin-neck that occurs post ATP hydrolysis during the transition from weakly to strongly actin-bound myosin states concomitant with P_i release and prior to ADP dissociation. The activating role of actin on product release and force generation is well documented; however, the communication paths associated with weak-to-strong transitions are poorly characterized. With the aid of mutant analyses based on kinetic investigations and simulations, we identified the W-helix as an important hub coupling the structural changes of switch elements during ATP hydrolysis to temporally controlled interactions with actin that are passed to the central transdu

SUBMITTER: Franz P 

PROVIDER: S-EPMC7795666 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature