3D Dissection of Structural Membrane-Wall Contacts in Filamentous Moss Protonemata.

ABSTRACT: In conventional light microscopy, the adjacent cell walls of filamentous moss protonemata are seen from its narrow side thereby obscuring the major area of cell-cell connection. Optical sectioning, segmentation and 3D reconstructions allow the tilting and rotation of intracellular structures thereby greatly improving our understanding of interaction between organelles, membranes and the cell wall. Often, the findings also allow for conclusions on the respective functions. The moss Physcomitrium (Physcomitrella) patens is a model organism for growth, development and morphogenesis. Its filamentous protonemata are ideal objects for microscopy. Here, we investigated the cell wall between two neighboring cells and the connection of membranes towards this wall after plasmolysis in 0.8 M mannitol

SUBMITTER: Harant D 

PROVIDER: S-EPMC7796084 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature