Specific and Sensitive Detection of Neuroblastoma mRNA Markers by Multiplex RT-qPCR.

ABSTRACT: mRNA RT-qPCR is shown to be a very sensitive technique to detect minimal residual disease (MRD) in patients with neuroblastoma. Multiple mRNA markers are known to detect heterogeneous neuroblastoma cells in bone marrow (BM) or blood from patients. However, the limited volumes of BM and blood available can hamper the detection of multiple markers. To make optimal use of these samples, we developed a multiplex RT-qPCR for the detection of MRD in neuroblastoma. GUSB and PHOX2B were tested as single markers. The adrenergic markers TH, GAP43, CHRNA3 and DBH and mesenchymal markers POSTN, PRRX1 and FMO3 were tested in multiplex. Using control blood and BM, we established new thresholds for positivity. Comparison of multiplex and sing

SUBMITTER: van Zogchel LMJ 

PROVIDER: S-EPMC7796198 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature