Deconstructing transcriptional variations and their effects on immunomodulatory function among human mesenchymal stromal cells.

ABSTRACT:

Background

Mesenchymal stromal cell (MSC)-based therapies are being actively investigated in various inflammatory disorders. However, functional variability among MSCs cultured in vitro will lead to distinct therapeutic efficacies. Until now, the mechanisms behind immunomodulatory functional variability in MSCs are still unclear.

Methods

We systemically investigated transcriptomic variations among MSC samples derived from multiple tissues to reveal their effects on immunomodulatory functions of MSCs. We then analyzed transcriptomic changes of MSCs licensed with INFγ to identify potential molecular mechanisms that result in distinct MSC samples with different immunomodulatory potency.

Results

MSCs were clustered into distinct groups showing different functional enrichm

SUBMITTER: Sun C 

PROVIDER: S-EPMC7796611 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature