In vitro 3D culture lung model from expanded primary cystic fibrosis human airway cells.

ABSTRACT:

Background

In vitro cystic fibrosis (CF) models are crucial for understanding the mechanisms and consequences of the disease. They are also the gold standard for pre-clinical efficacy studies of current and novel CF drugs. However, few studies have investigated expansion and differentiation of primary CF human bronchial epithelial (CF-HBE) cells. Here we describe culture conditions to expand primary CF airway cells while preserving their ability to differentiate into 3D epithelial cultures expressing functional cystic fibrosis transmembrane conductance regulator (CFTR) ion channels that responds to CFTR modulators.

Methods

Primary CF airway cells were expanded using PneumaCult^TM-Ex Plus (StemCell Technologies) medium with no feeder cells or added Rho kinase (ROCK)

SUBMITTER: Rayner RE 

PROVIDER: S-EPMC7796805 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature