Byproduct-free geraniol glycosylation by whole-cell biotransformation with recombinant Escherichia coli.

ABSTRACT:

Objective

Geraniol, a fragrance of great importance in the consumer goods industry, can be glucosylated by the UDP-glucose-dependent glucosyltransferase VvGT14a from Vitis vinifera, yielding more stable geranyl glucoside. Escherichia coli expressing VvGT14a is a convenient whole-cell biocatalyst for this biotransformation due to its intrinsic capability for UDP-glucose regeneration. The low water solubility and high cytotoxicity of geraniol can be overcome in a biphasic system where the non-aqueous phase functions as an in situ substrate reservoir. However, the effect of different process variables on the biphasic whole-cell biotransformation is unknown. Thus, the goal of this study was to identify potential bottlenecks during biotransformation with in situ geraniol supply via isop

SUBMITTER: Priebe X 

PROVIDER: S-EPMC7796880 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature