Simultaneous detection of multiple mRNAs and proteins in bovine IVD cells and tissue with single cell resolution.

ABSTRACT:

Objectives

Interactions of cells with their neighbors and influences by the surrounding extracellular matrix (ECM) is reflected in a cells transcriptome and proteome. In tissues comprised of heterogeneous cell populations or cells depending on ECM signalling cues such as those of the intervertebral disc (IVD), this information is obscured or lost when cells are pooled for the commonly used transcript analysis by quantitative PCR or RNA sequencing. Instead, these cells require means to analyse RNA transcript and protein distribution at a single cell or subcellular level to identify different cell types and functions, without removing them from their surrounding signalling cues.

Results

We developed a simple, sequential protocol combining RNA is situ hybridisation (RISH) and i

SUBMITTER: Li K 

PROVIDER: S-EPMC7796921 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature