Functional role of the long noncoding RNA X-inactive specific transcript in leiomyoma pathogenesis.

ABSTRACT:

Objective

To determine the expression and functional roles of a long noncoding RNA (lncRNA) X-inactive specific transcript (XIST) in leiomyoma.

Design

Experimental study.

Setting

Academic research laboratory.

Patient(s)

Women undergoing hysterectomy for leiomyoma.

Intervention(s)

Overexpression and underexpression of XIST; blockade of specific protein 1 (SP1).

Main outcome measure(s)

Expression of XIST in leiomyoma and its effects on microRNA 29c (miR-29c), miR-200c, and their targets.

Result(s)

Leiomyoma expressed statistically significantly more XIST as compared with matched myometrium, independent of race/ethnicity and menstrual cycle phase. By use of a three-dimensional spheroid culture system, we found reduced XIST levels in leiomyoma

SUBMITTER: Chuang TD 

PROVIDER: S-EPMC7796933 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature