Derivation of Airway Basal Stem Cells from Human Pluripotent Stem Cells.

ABSTRACT: The derivation of tissue-specific stem cells from human induced pluripotent stem cells (iPSCs) would have broad reaching implications for regenerative medicine. Here, we report the directed differentiation of human iPSCs into airway basal cells ("iBCs"), a population resembling the stem cell of the airway epithelium. Using a dual fluorescent reporter system (NKX2-1^GFP;TP63^tdTomato), we track and purify these cells as they first emerge as developmentally immature NKX2-1^GFP+ lung progenitors and subsequently augment a TP63 program during proximal airway epithelial patterning. In response to primary basal cell medium, NKX2-1^GFP+/TP63^tdTomato+ cells display the molecular and functional phenotype of airway basal cells, including the capacit

SUBMITTER: Hawkins FJ 

PROVIDER: S-EPMC7796997 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature