The versatile interactome of chloroplast ribosomes revealed by affinity purification mass spectrometry.

ABSTRACT: In plant cells, chloroplast gene expression is predominantly controlled through post-transcriptional regulation. Such fine-tuning is vital for precisely orchestrating protein complex assembly as for the photosynthesis machinery and for quickly responding to environmental changes. While regulation of chloroplast protein synthesis is of central importance, little is known about the degree and nature of the regulatory network, mainly due to challenges associated with the specific isolation of transient ribosome interactors. Here, we established a ribosome affinity purification method, which enabled us to broadly uncover putative ribosome-associated proteins in chloroplasts. Endogenously tagging of a protein of the large or small subunit revealed not only interactors of the holo complex, but a

SUBMITTER: Westrich LD 

PROVIDER: S-EPMC7797057 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature