Linc-SCRG1 accelerates progression of hepatocellular carcinoma as a ceRNA of miR26a to derepress SKP2.

ABSTRACT:

Background

Increasing evidence has demonstrated that long noncoding RNAs (lncRNAs) have regulatory functions in hepatocellular carcinoma (HCC). The link between lincSCRG1 and HCC remains unclear.

Methods

To explore the lincSCRG1 regulation axis, bioinformatics, RIP and luciferase reporter assay were performed. The expressions of lincSCRG1-miR26a-SKP2 were detected in HCC tissues and cell lines through qPCR and western blot. The functions of HCC cells were investigated through in vitro assays (MTT, colony formation, transwell and flow cytometry) and the inner effect of lincSCRG1-miR26a in vivo was evaluated by xenografts and liver metatstatic nude mice models.

Results

LincSCRG1 was found to be strongly elevated in human HCC tissues and cell lines. MiR26a and S phase ki

SUBMITTER: Hu JJ 

PROVIDER: S-EPMC7797122 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature