Split-root systems: detailed methodology, alternative applications, and implications at leaf proteome level.

ABSTRACT:

Background

Split-root systems (SRS) have many applications in plant sciences, but their implementation, depending on the experimental design, can be difficult and time-consuming. Additionally, the system is not exempt from limitations, since the time required for the establishment of the SRS imposes a limit to how early in plant development experiments can be performed. Here, we optimized and explained in detail a method for establishing a SRS in young Arabidopsis thaliana seedlings, both in vitro and in soil.

Results

We found that the partial de-rooting minimized the recovery time compared to total de-rooting, thus allowing the establishment of the split-root system in younger plants. Analysis of changes in the Arabidopsis leaf proteome following the de-rooting procedure hi

SUBMITTER: Saiz-Fernandez I 

PROVIDER: S-EPMC7797125 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature