M⁶A regulator-based methylation modification patterns characterized by distinct tumor microenvironment immune profiles in colon cancer.

ABSTRACT: Recent studies have highlighted the biological significance of RNA N⁶-methyladenosine (m⁶A) modification in tumorigenicity and progression. However, it remains unclear whether m⁶A modifications also have potential roles in immune regulation and tumor microenvironment (TME) formation. Methods: In this study, we curated 23 m⁶A regulators and performed consensus molecular subtyping with NMF algorithm to determine m⁶A modification patterns and the m⁶A-related gene signature in colon cancer (CC). The ssGSEA and CIBERSORT algorithms were employed to quantify the relative infiltration levels of various immune cell subsets. An PCA algorithm based m⁶Sig scoring scheme was used to evaluate the m⁶A modifica

SUBMITTER: Chong W 

PROVIDER: S-EPMC7797678 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature