Measurement of mitochondrial respiration in adherent cells by Seahorse XF96 Cell Mito Stress Test.
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ABSTRACT:
SUBMITTER: Gu X
PROVIDER: S-EPMC7797920 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
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Measurement of mitochondrial respiration in adherent cells by Seahorse XF96 Cell Mito Stress Test.
Gu Xia X Ma Yuan Y Liu Yi Y Wan Qiang Q
STAR protocols 20201230 1
Mitochondria play pivotal roles in cellular energy metabolism. Most of the intracellular adenosine triphosphate (ATP) is generated by mitochondrial respiration. The Cell Mito Stress Test is a common method to measure the key parameters of mitochondrial respiration. Here, we use the human cell line HK-2 as an example to present the procedures to quantify the oxygen consumption rate using a Seahorse XFe96 extracellular flux analyzer. For complete details on the use and execution of this protocol, ...[more]
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