NanoSIMS observations of mouse retinal cells reveal strict metabolic controls on nitrogen turnover.

ABSTRACT:

Background

Most of the cells of the mammalian retina are terminally differentiated, and do not regenerate once fully developed. This implies that these cells have strict controls over their metabolic processes, including protein turnover. We report the use of metabolic labelling procedures and secondary ion mass spectrometry imaging to examine nitrogen turnover in retinal cells, with a focus on the outer nuclear layer, inner nuclear layer, and outer plexiform layer.

Results

We find that turnover can be observed in all cells imaged using NanoSIMS. However, the rate of turnover is not constant, but varies between different cellular types and cell regions. In the inner and outer nuclear layers, turnover rate is higher in the cytosol than in the nucleus of each cell. Turnover ra

SUBMITTER: Bonnin EA 

PROVIDER: S-EPMC7798281 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature