Discovery of natural products capable of inducing porcine host defense peptide gene expression using cell-based high throughput screening.

ABSTRACT:

Background

In-feed antibiotics are being phased out in livestock production worldwide. Alternatives to antibiotics are urgently needed to maintain animal health and production performance. Host defense peptides (HDPs) are known for their broad-spectrum antimicrobial and immunomodulatory capabilities. Enhancing the synthesis of endogenous HDPs represents a promising antibiotic alternative strategy to disease control and prevention.

Methods

To identify natural products with an ability to stimulate the synthesis of endogenous HDPs, we performed a high-throughput screening of 1261 natural products using a newly-established stable luciferase reporter cell line known as IPEC-J2/pBD3-luc. The ability of the hit compounds to induce HDP genes in porcine IPEC-J2 intestinal epithelial

SUBMITTER: Wang J 

PROVIDER: S-EPMC7798283 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature