Development of a real-time quantitative PCR method for detection and quantification of Prevotella copri.

ABSTRACT:

Background

Since its discovery in 2007, the importance of the human gut bacterium Prevotella copri (P. copri) has been widely recognized with its links to diet and health status and potential as next generation probiotic. Therefore, precise, convenient and cost-effective diagnostic tools for the detection and quantification of P. copri from clinical and environmental samples are needed.

Results

In this study, a Sybr Green qPCR protocol for P. copri detection and quantification was developed and tested on P. copri-spiked murine faeces samples targeting both the 16S rRNA gene and P. copri genome specific genes. The use of one 16S rRNA primer pair and 2 genome specific primer pairs resulted in at least 10x higher specificity and sensitivity than the primer-only PCR currently ci

SUBMITTER: Verbrugghe P 

PROVIDER: S-EPMC7798335 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature