Kβ X-ray Emission Spectroscopy as a Probe of Cu(I) Sites: Application to the Cu(I) Site in Preprocessed Galactose Oxidase.

ABSTRACT: Cu(I) active sites in metalloproteins are involved in O₂ activation, but their O₂ reactivity is difficult to study due to the Cu(I) d¹⁰ closed shell which precludes the use of conventional spectroscopic methods. Kβ X-ray emission spectroscopy (XES) is a promising technique for investigating Cu(I) sites as it detects photons emitted by electronic transitions from occupied orbitals. Here, we demonstrate the utility of Kβ XES in probing Cu(I) sites in model complexes and a metalloprotein. Using Cu(I)Cl, emission features from double-ionization (DI) states are identified using varying incident X-ray photon energies, and a reasonable method to correct the data to remove DI contributions is presented. Kβ XES spectra of Cu(I) model complexes, having biologically r

SUBMITTER: Lim H 

PROVIDER: S-EPMC7799175 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature