Ballistic Labeling of Pyramidal Neurons in Brain Slices and in Primary Cell Culture.

ABSTRACT: It has been reported that the size and shape of dendritic spines is related to their structural plasticity. To identify the morphological structure of pyramidal neurons and dendritic spines, a ballistic labeling technique can be utilized. In the present protocol, pyramidal neurons are labeled with DilC18(3) dye and analyzed using neuronal reconstruction software to assess neuronal morphology and dendritic spines. To investigate neuronal structure, dendritic branching analysis and Sholl analysis are performed, allowing researchers to draw inferences about dendritic branching complexity and neuronal arbor complexity, respectively. The evaluation of dendritic spines is conducted using an automatic assisted classification algorithm integral to the reconstruction software, which classifies spin

SUBMITTER: Li H 

PROVIDER: S-EPMC7799180 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature