Platelets express adaptor proteins of the extrinsic apoptosis pathway and can activate caspase-8.

ABSTRACT:

Background

Apoptotic pathways in platelets are important for their survival and function. Platelet apoptosis may be involved in the pathogenesis of immune thrombocytopenia (ITP), an autoimmune-mediated disease. In contrast to the intrinsic apoptosis pathway, not much is known about the extrinsic pathway mechanisms in platelets.

Objectives

To investigate the expression of proteins involved in the extrinsic apoptosis pathway, including the death receptors, adaptor and regulator proteins in human platelets. To determine a possible trigger of the extrinsic apoptosis pathway in platelets.

Methods

To investigate the expression of key markers of the extrinsic pathway we used targeted immunofluorescence and flow cytometry assays. To study their expression and interaction we p

SUBMITTER: Goelz N 

PROVIDER: S-EPMC7799768 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature