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Mitochondrial genome of Tesseropora rosea: molecular evidence for non-monophyly of the genus Tetraclita.

ABSTRACT: The complete mitochondrial genome of Tesseropora rosea (Tetraclitidae) was presented. The genome is a circular molecule of 15,330?bp, which encodes 13 PCGs, two rRNA genes, and 22 tRNA genes. The length of all non-coding regions is 768?bp, with the longest one speculated as the control region (255?bp), which is located between 12S rRNA and trnK. Phylogenetic analysis based on mitochondrial PCGs shows that T. rosea is nested within the genus Tetraclita, more closely related to Tetraclita japonica than to Tetraclita rufotincta or Tetraclita serrata. Thus, the monophyly of the genus Tetraclita is not supported.

SUBMITTER: Cai YF

PROVIDER: S-EPMC7800120 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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Mitochondrial genome of Tesseropora rosea: molecular evidence for non-monophyly of the genus Tetraclita.

Cai Yue Feng YF Shen Xin X Zhou Liang L Chu Ka Hou KH Chan Benny Kwok Kan BKK

Mitochondrial DNA. Part B, Resources 20180105 1

The complete mitochondrial genome of Tesseropora rosea (Tetraclitidae) was presented. The genome is a circular molecule of 15,330 bp, which encodes 13 PCGs, two rRNA genes, and 22 tRNA genes. The length of all non-coding regions is 768 bp, with the longest one speculated as the control region (255 bp), which is located between 12S rRNA and trnK. Phylogenetic analysis based on mitochondrial PCGs shows that T. rosea is nested within the genus Tetraclita, more clo ...[more]

PMID: 33474078

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Project description:BackgroundThe orders Ascaridida, Oxyurida, and Spirurida represent major components of zooparasitic nematode diversity, including many species of veterinary and medical importance. Phylum-wide nematode phylogenetic hypotheses have mainly been based on nuclear rDNA sequences, but more recently complete mitochondrial (mtDNA) gene sequences have provided another source of molecular information to evaluate relationships. Although there is much agreement between nuclear rDNA and mtDNA phylogenies, relationships among certain major clades are different. In this study we report that mtDNA sequences do not support the monophyly of Ascaridida, Oxyurida and Spirurida (clade III) in contrast to results for nuclear rDNA. Results from mtDNA genomes show promise as an additional independently evolving genome for developing phylogenetic hypotheses for nematodes, although substantially increased taxon sampling is needed for enhanced comparative value with nuclear rDNA. Ultimately, topological incongruence (and congruence) between nuclear rDNA and mtDNA phylogenetic hypotheses will need to be tested relative to additional independent loci that provide appropriate levels of resolution.ResultsFor this comparative phylogenetic study, we determined the complete mitochondrial genome sequences of three nematode species, Cucullanus robustus (13,972 bp) representing Ascaridida, Wellcomia siamensis (14,128 bp) representing Oxyurida, and Heliconema longissimum (13,610 bp) representing Spirurida. These new sequences were used along with 33 published nematode mitochondrial genomes to investigate phylogenetic relationships among chromadorean orders. Phylogenetic analyses of both nucleotide and amino acid sequence datasets support the hypothesis that Ascaridida is nested within Rhabditida. The position of Oxyurida within Chromadorea varies among analyses; in most analyses this order is sister to the Ascaridida plus Rhabditida clade, with representative Spirurida forming a distinct clade, however, in one case Oxyurida is sister to Spirurida. Ascaridida, Oxyurida, and Spirurida (the sampled clade III taxa) do not form a monophyletic group based on complete mitochondrial DNA sequences. Tree topology tests revealed that constraining clade III taxa to be monophyletic, given the mtDNA datasets analyzed, was a significantly worse result.ConclusionThe phylogenetic hypotheses from comparative analysis of the complete mitochondrial genome data (analysis of nucleotide and amino acid datasets, and nucleotide data excluding 3rd positions) indicates that nematodes representing Ascaridida, Oxyurida and Spirurida do not share an exclusive most recent common ancestor, in contrast to published results based on nuclear ribosomal DNA. Overall, mtDNA genome data provides reliable support for nematode relationships that often corroborates findings based on nuclear rDNA. It is anticipated that additional taxonomic sampling will provide a wealth of information on mitochondrial genome evolution and sequence data for developing phylogenetic hypotheses for the phylum Nematoda.

Dataset Information

Mitochondrial genome of Tesseropora rosea: molecular evidence for non-monophyly of the genus Tetraclita.

Publications

Mitochondrial genome of <i>Tesseropora rosea</i>: molecular evidence for non-monophyly of the genus <i>Tetraclita</i>.

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