Dataset Information

Assembly and phylogenetic analysis of complete chloroplast genome of a wild tobacco Nicotiana Attenuata.

ABSTRACT: Nicotiana attenuata (N. attenuata) is an annual herb that induces nicotine, volatiles and phenolics in response to damage. In this study, we assembled the complete chloroplast (cp) genome of N. attenuata with the length of 155,886 bp, which was composed of a large single copy region (LSC) of 86,602 bp, a small copy (SSC) region of 18,518 bp and a pair of inverted repeats (IR) of 25,383 bp. This typical cp genome contains 112 unique genes, including 78 protein-coding genes, 30 tRNA genes and four rRNA genes. Phylogenetic analysis of 32 plant cp genomes based on 76 conserved protein-coding genes shows that N. attenuata is evolutionarily close to N. tabacum, then Capsicum annuum. The complete cp genome sequence of N. attenuata may provide a crucial foundation for the study of evolution, molecular biology and genetic engineering in Nicotiana species.

SUBMITTER: Lin H

PROVIDER: S-EPMC7800318 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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Assembly and phylogenetic analysis of complete chloroplast genome of a wild tobacco Nicotiana Attenuata.

Lin Haifeng H Bai Di D

Mitochondrial DNA. Part B, Resources 20171106 2

Nicotiana attenuata (N. attenuata) is an annual herb that induces nicotine, volatiles and phenolics in response to damage. In this study, we assembled the complete chloroplast (cp) genome of N. attenuata with the length of 155,886 bp, which was composed of a large single copy region (LSC) of 86,602 bp, a small copy (SSC) region of 18,518 bp and a pair of inverted repeats (IR) of 25,383 bp. This typical cp genome contains 112 unique genes, including 78 protein-coding genes, 3 ...[more]

PMID: 33490477

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Project description:BackgroundNicotiana attenuata (coyote tobacco) is an ecological model for studying plant-environment interactions and plant gene function under real-world conditions. During the last decade, large amounts of genomic, transcriptomic and metabolomic data have been generated with this plant which has provided new insights into how native plants interact with herbivores, pollinators and microbes. However, an integrative and open access platform that allows for the efficient mining of these -omics data remained unavailable until now.DescriptionWe present the Nicotiana attenuata Data Hub (NaDH) as a centralized platform for integrating and visualizing genomic, phylogenomic, transcriptomic and metabolomic data in N. attenuata. The NaDH currently hosts collections of predicted protein coding sequences of 11 plant species, including two recently sequenced Nicotiana species, and their functional annotations, 222 microarray datasets from 10 different experiments, a transcriptomic atlas based on 20 RNA-seq expression profiles and a metabolomic atlas based on 895 metabolite spectra analyzed by mass spectrometry. We implemented several visualization tools, including a modified version of the Electronic Fluorescent Pictograph (eFP) browser, co-expression networks and the Interactive Tree Of Life (iTOL) for studying gene expression divergence among duplicated homologous. In addition, the NaDH allows researchers to query phylogenetic trees of 16,305 gene families and provides tools for analyzing their evolutionary history. Furthermore, we also implemented tools to identify co-expressed genes and metabolites, which can be used for predicting the functions of genes. Using the transcription factor NaMYB8 as an example, we illustrate that the tools and data in NaDH can facilitate identification of candidate genes involved in the biosynthesis of specialized metabolites.ConclusionThe NaDH provides interactive visualization and data analysis tools that integrate the expression and evolutionary history of genes in Nicotiana, which can facilitate rapid gene discovery and comparative genomic analysis. Because N. attenuata shares many genome-wide features with other Nicotiana species including cultivated tobacco, and hence NaDH can be a resource for exploring the function and evolution of genes in Nicotiana species in general. The NaDH can be accessed at: http://nadh.ice.mpg.de/ .

Dataset Information

Assembly and phylogenetic analysis of complete chloroplast genome of a wild tobacco Nicotiana Attenuata.

Publications

Assembly and phylogenetic analysis of complete chloroplast genome of a wild tobacco <i>Nicotiana Attenuata</i>.

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