Dataset Information

Species identification of juvenile fishes of the genus Pseudoblennius using mitochondrial DNA barcoding.

ABSTRACT: Species identification is important in natural science and should be precise. Six specimens of juvenile Pseudoblennius were collected from the eastern coastal waters of the Korean Peninsula and Jeju Island in 2016-2017, and identified for the first time using DNA barcoding based on mitochondrial DNA cytochrome oxidase subunit I sequences. DNA barcoding analysis supported three adult species of genus Pseudoblennius (P. cottoides, P. marmoratus, and P. percoides) being quite distinct from each other. Six juvenile specimens were completely identified: two as P. cottoides; two more as P. marmoratus; and the final two as P. percoides. Mitochondrial DNA COI can be effective as a means of species identification method for the genus Pseudoblennius.

SUBMITTER: Kwun HJ

PROVIDER: S-EPMC7800764 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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Species identification of juvenile fishes of the genus Pseudoblennius using mitochondrial DNA barcoding.

Kwun Hyuck Joon HJ

Mitochondrial DNA. Part B, Resources 20180328 1

Species identification is important in natural science and should be precise. Six specimens of juvenile Pseudoblennius were collected from the eastern coastal waters of the Korean Peninsula and Jeju Island in 2016-2017, and identified for the first time using DNA barcoding based on mitochondrial DNA cytochrome oxidase subunit I sequences. DNA barcoding analysis supported three adult species of genus Pseudoblennius (P. cottoides, P. marmoratus, and P. percoides) ...[more]

PMID: 33474185

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Project description:BackgroundDNA barcoding enhances the prospects for species-level identifications globally using a standardized and authenticated DNA-based approach. Reference libraries comprising validated DNA barcodes (COI) constitute robust datasets for testing query sequences, providing considerable utility to identify marine fish and other organisms. Here we test the feasibility of using DNA barcoding to assign species to tissue samples from fish collected in the central Mediterranean Sea, a major contributor to the European marine ichthyofaunal diversity.Methodology/principal findingsA dataset of 1278 DNA barcodes, representing 218 marine fish species, was used to test the utility of DNA barcodes to assign species from query sequences. We tested query sequences against 1) a reference library of ranked DNA barcodes from the neighbouring North East Atlantic, and 2) the public databases BOLD and GenBank. In the first case, a reference library comprising DNA barcodes with reliability grades for 146 fish species was used as diagnostic dataset to screen 486 query DNA sequences from fish specimens collected in the central basin of the Mediterranean Sea. Of all query sequences suitable for comparisons 98% were unambiguously confirmed through complete match with reference DNA barcodes. In the second case, it was possible to assign species to 83% (BOLD-IDS) and 72% (GenBank) of the sequences from the Mediterranean. Relatively high intraspecific genetic distances were found in 7 species (2.2%-18.74%), most of them of high commercial relevance, suggesting possible cryptic species.Conclusion/significanceWe emphasize the discriminatory power of COI barcodes and their application to cases requiring species level resolution starting from query sequences. Results highlight the value of public reference libraries of reliability grade-annotated DNA barcodes, to identify species from different geographical origins. The ability to assign species with high precision from DNA samples of disparate quality and origin has major utility in several fields, from fisheries and conservation programs to control of fish products authenticity.

Dataset Information

Species identification of juvenile fishes of the genus Pseudoblennius using mitochondrial DNA barcoding.

Publications

Species identification of juvenile fishes of the genus <i>Pseudoblennius</i> using mitochondrial DNA barcoding.

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