Dataset Information

The complete mitochondrial genome sequences of two Isospora species (Eimeriidae, Eucoccidiorida, Coccidiasina, Apicomplexa) causing coccidiosis in superb glossy starlings, Lamprotornis superbus (Aves: Sturnidae).

ABSTRACT: Complete mitochondrial genomes are reported for two Isospora species causing systemic coccidiosis in Superb Glossy Starlings (Aves: Sturnidae). The A/T rich (34.7% G/C) genomes were 6223 bp in length for Isospora greineri and 6217 bp for Isospora superbusi. Each encoded 3 protein-coding genes, (COI, COIII and CytB) plus 18 LSU and 14 SSU rDNA fragments. Arrangement of protein- and rRNA-coding regions was identical to known Eimeria sp. mt genomes; start codon usage was conventional. The mitochondrial genome structures of Isospora and Eimeria species are conserved and reflect the close phylogenetic association between these eimeriid genera of apicomplexan parasites.

SUBMITTER: Hafeez MA

PROVIDER: S-EPMC7800974 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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Publications

The complete mitochondrial genome sequences of two Isospora species (Eimeriidae, Eucoccidiorida, Coccidiasina, Apicomplexa) causing coccidiosis in superb glossy starlings, Lamprotornis superbus (Aves: Sturnidae).

Hafeez Mian A MA Barta John R JR

Mitochondrial DNA. Part B, Resources 20171127 2

Complete mitochondrial genomes are reported for two Isospora species causing systemic coccidiosis in Superb Glossy Starlings (Aves: Sturnidae). The A/T rich (34.7% G/C) genomes were 6223 bp in length for Isospora greineri and 6217 bp for Isospora superbusi. Each encoded 3 protein-coding genes, (COI, COIII and CytB) plus 18 LSU and 14 SSU rDNA fragments. Arrangement of protein- and rRNA-coding regions was identical to known Eimeria sp. mt genomes; start codon usage was ...[more]

PMID: 33490480

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Project description:Anadromous alewives, Alosa pseudoharengus, have experienced significant population level declines caused by factors including habitat destruction. Alewives occur in two different life histories, anadromous and landlocked forms. The landlocked alewife evolved from ancestral anadromous populations, resulting in an exclusively freshwater and phenotypically unique form. The occurrence of parasites in a host is linked to the environment, making alewives an ideal model to compare parasitology within a single species with contrasting life histories. Currently, little information exists on the presence and impacts of parasites in these fish populations; the present study sets out to better understand coccidiosis in the threatened anadromous populations and to understand how coccidian parasites compare in both life history forms. The intestinal coccidian, Goussia ameliae n. sp., was described infecting the pyloric cecum of 76% and 86% of young-of-the-year and adult anadromous alewives, respectively, from the Maurice River, New Jersey, USA. The coccidian was found in landlocked alewife populations with a prevalence of 92% and 34% in YOY and adult fish, respectively. An analysis of the small subunit 18S ribosomal RNA gene of G. ameliae from both life history forms demonstrated that the coccidian had 100% sequence identity, confirming the same parasite species in both forms. Though genetic analysis demonstrated G. ameliae to be identical, some differences were observed in sporulation and morphology of the parasite within the two populations. The sporocysts in anadromous populations were shorter and wider, and sporulation timing differed from that of landlocked fish. These differences may either be attributed to differences in the host type or to the sporulation environment. Lastly, alewives from landlocked populations were frequently co-infected with a second coccidian species in the posterior intestine, which occurred at a lower prevalence. This species, G. alosii n. sp., was described based on morphological characters of the sporulated oocysts in fresh parasitological preparations.

Project description:BackgroundClinical and subclinical coccidiosis is cosmopolitan and inflicts significant losses to the poultry industry globally. Seven named Eimeria species are responsible for coccidiosis in turkeys: Eimeria dispersa; Eimeria meleagrimitis; Eimeria gallopavonis; Eimeria meleagridis; Eimeria adenoeides; Eimeria innocua; and, Eimeria subrotunda. Although attempts have been made to characterize these parasites molecularly at the nuclear 18S rDNA and ITS loci, the maternally-derived and mitotically replicating mitochondrial genome may be more suited for species level molecular work; however, only limited sequence data are available for Eimeria spp. infecting turkeys. The purpose of this study was to sequence and annotate the complete mitochondrial genomes from 5 Eimeria species that commonly infect the domestic turkey (Meleagris gallopavo).MethodsSix single-oocyst derived cultures of five Eimeria species infecting turkeys were PCR-amplified and sequenced completely prior to detailed annotation. Resulting sequences were aligned and used in phylogenetic analyses (BI, ML, and MP) that included complete mitochondrial genomes from 16 Eimeria species or concatenated CDS sequences from each genome.ResultsComplete mitochondrial genome sequences were obtained for Eimeria adenoeides Guelph, 6211 bp; Eimeria dispersa Briston, 6238 bp; Eimeria meleagridis USAR97-01, 6212 bp; Eimeria meleagrimitis USMN08-01, 6165 bp; Eimeria gallopavonis Weybridge, 6215 bp; and Eimeria gallopavonis USKS06-01, 6215 bp). The order, orientation and CDS lengths of the three protein coding genes (COI, COIII and CytB) as well as rDNA fragments encoding ribosomal large and small subunit rRNA were conserved among all sequences. Pairwise sequence identities between species ranged from 88.1% to 98.2%; sequence variability was concentrated within CDS or between rDNA fragments (where indels were common). No phylogenetic reconstruction supported monophyly of Eimeria species infecting turkeys; Eimeria dispersa may have arisen via host switching from another avian host. Phylogenetic analyses suggest E. necatrix and E. tenella are related distantly to other Eimeria of chickens.ConclusionsMitochondrial genomes of Eimeria species sequenced to date are highly conserved with regard to gene content and structure. Nonetheless, complete mitochondrial genome sequences and, particularly the three CDS, possess sufficient sequence variability for differentiating Eimeria species of poultry. The mitochondrial genome sequences are highly suited for molecular diagnostics and phylogenetics of coccidia and, potentially, genetic markers for molecular epidemiology.

Dataset Information

The complete mitochondrial genome sequences of two Isospora species (Eimeriidae, Eucoccidiorida, Coccidiasina, Apicomplexa) causing coccidiosis in superb glossy starlings, Lamprotornis superbus (Aves: Sturnidae).

Publications

The complete mitochondrial genome sequences of two <i>Isospora</i> species (Eimeriidae, Eucoccidiorida, Coccidiasina, Apicomplexa) causing coccidiosis in superb glossy starlings, <i>Lamprotornis superbus</i> (Aves: Sturnidae).

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