Enterococcus faecalis shifts macrophage polarization toward M1-like phenotype with an altered cytokine profile.

ABSTRACT: Background: The macrophage is an innate immune defense cell involved in pathogen recognition and clearance. Aim: In view of the diversity of the macrophage phenotype and function, the present study investigated how Enterococcus faecalis infection affects the differentiation, phenotype and cytokine profile of macrophages. Methods: Murine bone marrow-derived stem cells were co-cultured with E. faecalis before and after differentiation. Macrophage M0 polarization towards M1 or M2 was initiated at day 6 by addition of LPS and INF-γ, or IL-4 and IL-13, respectively. Results: E. faecalis did not inhibit macrophage differentiation and were identified within macrophages. Viability of the macrophages infected with E. faecalis prior to differen

SUBMITTER: Mohamed Elashiry M 

PROVIDER: S-EPMC7801083 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature