Carbon dioxide inhibits UVB-induced inflammatory response by activating the proton-sensing receptor, GPR65, in human keratinocytes.

ABSTRACT: Carbon dioxide (CO₂) is the predominant gas molecule emitted during aerobic respiration. Although CO₂ can improve blood circulation in the skin via its vasodilatory effects, its effects on skin inflammation remain unclear. The present study aimed to examine the anti-inflammatory effects of CO₂ in human keratinocytes and skin. Keratinocytes were cultured under 15% CO₂, irradiated with ultraviolet B (UVB), and their inflammatory cytokine production was analyzed. Using multiphoton laser microscopy, the effect of CO₂ on pH was observed by loading a three-dimensional (3D)-cultured epidermis with a high-CO₂ concentration formulation. Finally, the effect of CO₂ on UVB-induced erythema was confirmed. CO₂ suppre

SUBMITTER: Sayama K 

PROVIDER: S-EPMC7801444 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature