Homotypic CARD-CARD interaction is critical for the activation of NLRP1 inflammasome.

ABSTRACT: Cytosolic inflammasomes are supramolecular complexes that are formed in response to intracellular pathogens and danger signals. However, as to date, the detailed description of a homotypic caspase recruitment domain (CARD) interaction between NLRP1 and ASC has not been presented. We found the CARD-CARD interaction between purified NLRP1^CARD and ASC^CARD experimentally and the filamentous supramolecular complex formation in an in vitro proteins solution. Moreover, we determined a high-resolution crystal structure of the death domain fold of the human ASC^CARD. Mutational and structural analysis revealed three conserved interfaces of the death domain superfamily (Type I, II, and III), which mediate the assembly of the NLRP1^CARD/ASC^CARD com

SUBMITTER: Xu Z 

PROVIDER: S-EPMC7801473 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature