Cryo-EM structure of Helicobacter pylori urease with an inhibitor in the active site at 2.0 A resolution.

ABSTRACT: Infection of the human stomach by Helicobacter pylori remains a worldwide problem and greatly contributes to peptic ulcer disease and gastric cancer. Without active intervention approximately 50% of the world population will continue to be infected with this gastric pathogen. Current eradication, called triple therapy, entails a proton-pump inhibitor and two broadband antibiotics, however resistance to either clarithromycin or metronidazole is greater than 25% and rising. Therefore, there is an urgent need for a targeted, high-specificity eradication drug. Gastric infection by H. pylori depends on the expression of a nickel-dependent urease in the cytoplasm of the bacteria. Here, we report the 2.0 Å resolution structure of the 1.1 MDa urease in complex with an inhibitor by cryo-electron mi

SUBMITTER: Cunha ES 

PROVIDER: S-EPMC7801526 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature