Ethanol exposure drives colon location specific cell composition changes in a normal colon crypt 3D organoid model.

ABSTRACT: Alcohol is a consistently identified risk factor for colon cancer. However, the molecular mechanism underlying its effect on normal colon crypt cells remains poorly understood. We employed RNA-sequencing to asses transcriptomic response to ethanol exposure (0.2% vol:vol) in 3D organoid lines derived from healthy colon (n = 34). Paired regression analysis identified 2,162 differentially expressed genes in response to ethanol. When stratified by colon location, a far greater number of differentially expressed genes were identified in organoids derived from the left versus right colon, many of which corresponded to cell-type specific markers. To test the hypothesis that the effects of ethanol treatment on colon organoid populations were in part due to differential cell composition, we incorpo

SUBMITTER: Devall M 

PROVIDER: S-EPMC7801615 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature