Mechanical Stability of a Small, Highly-Luminescent Engineered Protein NanoLuc.

ABSTRACT: NanoLuc is a bioluminescent protein recently engineered for applications in molecular imaging and cellular reporter assays. Compared to other bioluminescent proteins used for these applications, like Firefly Luciferase and Renilla Luciferase, it is ~150 times brighter, more thermally stable, and smaller. Yet, no information is known with regards to its mechanical properties, which could introduce a new set of applications for this unique protein, such as a novel biomaterial or as a substrate for protein activity/refolding assays. Here, we generated a synthetic NanoLuc derivative protein that consists of three connected NanoLuc proteins flanked by two human titin I91 domains on each side and present our mechanical studies at the single molecule level by performing Single Molecule Force Spec

SUBMITTER: Ding Y 

PROVIDER: S-EPMC7801952 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature