Small Molecule Inhibitors of the Bacterioferritin (BfrB)-Ferredoxin (Bfd) Complex Kill Biofilm-Embedded Pseudomonas aeruginosa Cells.

ABSTRACT: Bacteria depend on a well-regulated iron homeostasis to survive adverse environments. A key component of the iron homeostasis machinery is the compartmentalization of Fe³⁺ in bacterioferritin and its subsequent mobilization as Fe²⁺ to satisfy metabolic requirements. In Pseudomonas aeruginosa Fe³⁺ is compartmentalized in bacterioferritin (BfrB), and its mobilization to the cytosol requires binding of a ferredoxin (Bfd) to reduce the stored Fe³⁺ and release the soluble Fe²⁺. Blocking the BfrB-Bfd complex in P. aeruginosa by deletion of the bfd gene triggers an irreversible accumulation of Fe³⁺ in BfrB, concomitant cytosolic iron deficiency and significant impairment of biofilm development. Herein we report

SUBMITTER: Soldano A 

PROVIDER: S-EPMC7802073 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature