Global histone protein surface accessibility in yeast indicates a uniformly loosely packed genome with canonical nucleosomes.

ABSTRACT:

Background

The vast majority of methods available to characterize genome-wide chromatin structure exploit differences in DNA accessibility to nucleases or chemical crosslinking. We developed a novel method to gauge genome-wide accessibility of histone protein surfaces within nucleosomes by assessing reactivity of engineered cysteine residues with a thiol-specific reagent, biotin-maleimide (BM).

Results

Yeast nuclei were obtained from cells expressing the histone mutant H2B S116C, in which a cysteine resides near the center of the external flat protein surface of the nucleosome. BM modification revealed that nucleosomes are generally equivalently accessible throughout the S. cerevisiae genome, including heterochromatic regions, suggesting limited, higher-order chromatin struc

SUBMITTER: Marr LT 

PROVIDER: S-EPMC7802155 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature