As a biomarker for gastric cancer, circPTPN22 regulates the progression of gastric cancer through the EMT pathway.

ABSTRACT:

Background

Gastric cancer (GC) is one of the most common cancers in the world. Due to the lack of specific symptoms, more than 80% of patients are diagnosed as the advanced stage with a high mortality rate, so the early diagnosis of GC is incredibly essential. Circular RNAs (CircRNAs) are a kind of endogenous non-coding RNA with stable structure, the long half-life, and tumor specificity. It can be used as a diagnostic marker for tumors.

Method

Using circRNA sequencing technology screened three pairs of GC and adjacent tissues, and circRNAs with significant expression differences were screened out. The circular structure and characteristics of circPTPN22 were determined by RT-qPCR, agarose gel electrophoresis, Sanger sequencing, RNase R, and actinomycin D assays. Cell Counti

SUBMITTER: Ma S 

PROVIDER: S-EPMC7802183 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature