NGS-PrimerPlex: High-throughput primer design for multiplex polymerase chain reactions.

ABSTRACT: Multiplex polymerase chain reaction (PCR) has multiple applications in molecular biology, including developing new targeted next-generation sequencing (NGS) panels. We present NGS-PrimerPlex, an efficient and versatile command-line application that designs primers for different refined types of amplicon-based genome target enrichment. It supports nested and anchored multiplex PCR, redistribution among multiplex reactions of primers constructed earlier, and extension of existing NGS-panels. The primer design process takes into consideration the formation of secondary structures, non-target amplicons between all primers of a pool, primers and high-frequent genome single-nucleotide polymorphisms (SNPs) overlapping. Moreover, users of NGS-PrimerPlex are free from manually defining input genome

SUBMITTER: Kechin A 

PROVIDER: S-EPMC7802936 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature