Establishment of Etv5 gene knockout mice as a recipient model for spermatogonial stem cell transplantation.

ABSTRACT: Spermatogonial stem cell (SSC) transplantation is an alternative reproductive method to achieve conservation and production of elite animals in livestock production. Creating a recipient animal without endogenous germ cells is important for effective SSC transplantation. However, natural mutants with depletion of SSCs are difficult to obtain, and drug ablation of endogenous germ cells is arduous to perform for practical use. In this study, we used mouse models to study the preparation of recipients with congenital germ cell ablation. We knocked out (KO) Ets-variant gene 5 (Etv5) in mice using the CRISPR/Cas9 system. The testicular weight of Etv5^-/- mice was significantly lower than that of wild-type (WT) mice. The germ cell layer of the seminiferous tubules gradual

SUBMITTER: Zhang X 

PROVIDER: S-EPMC7803462 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature