Comparison of RNA m⁶A and DNA methylation profiles between mouse female germline stem cells and STO cells.

ABSTRACT: N⁶-methyladenosine (m⁶A) methylation modification is the most prevalent and abundant internal modification of eukaryotic mRNAs. Increasing evidence has shown that mRNA m⁶A plays important roles in the development of stem cells. However, to the best of our knowledge, no reports about the roles of mRNA m⁶A in mouse female germline stem cells (mFGSCs) have been published. In this study, we compared the genome-wide profiles of mRNA m⁶A methylation and DNA methylation between FGSCs and sandosinbred mice (SIM) embryo-derived thioguanine and ouabain-resistant (STO) cells. qRT-PCR revealed that the expression levels of mRNA m⁶A-related genes (Mettl3, Alkbh5, Ythdf1, Ythdf2, Ythdc1, and Ythdc2

SUBMITTER: Zhao X 

PROVIDER: S-EPMC7803632 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature