Oxidized Low-Density Lipoprotein Drives Dysfunction of the Liver Lymphatic System.

ABSTRACT:

Background and aims

As the incidence of nonalcoholic steatohepatitis (NASH) continues to rise, understanding how normal liver functions are affected during disease is required before developing novel therapeutics which could reduce morbidity and mortality. However, very little is understood about how the transport of proteins and cells from the liver by the lymphatic vasculature is affected by inflammatory mediators or during disease.

Methods

To answer these questions, we utilized a well-validated mouse model of NASH and exposure to highly oxidized low density lipoprotein (oxLDL). In addition to single cell sequencing, multiplexed immunofluorescence and metabolomic analysis of liver lymphatic endothelial cells (LEC)s we evaluated lymphatic permeability and transport both in 

SUBMITTER: Burchill MA 

PROVIDER: S-EPMC7803659 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature