Empirical evidence for concerted evolution in the 18S rDNA region of the planktonic diatom genus Chaetoceros.

ABSTRACT: Concerted evolution is a process of homogenisation of repetitive sequences within a genome through unequal crossing over and gene conversion. This homogenisation is never fully achieved because mutations always create new variants. Classically, concerted evolution has been detected as "noise" in electropherograms and these variants have been characterised through cloning and sequencing of subsamples of amplified products. However, this approach limits the number of detectable variants and provides no information about the abundance of each variant. In this study, we investigated concerted evolution by using environmental time-series metabarcoding data, single strain high-throughput sequencing (HTS) and a collection of Sanger reference barcode sequences. We used six species of the marine pl

SUBMITTER: De Luca D 

PROVIDER: S-EPMC7804092 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature