The RNA methyltransferase NSUN6 suppresses pancreatic cancer development by regulating cell proliferation.

ABSTRACT:

Background

Pancreatic cancer (PC) is one of the most lethal solid malignancies in the world due to its excessive cell proliferation and aggressive metastatic features. Emerging evidences revealed the importance of posttranscriptional modifications of RNAs in PC progression. However, knowledge about the 5-methylcytosine (m5C) RNA modification in PC is still extremely limited. In this study, we attempted to explore the expression changes and clinical significances of 12 known m5C-related genes among PC patients.

Methods

A total of 362 normal and 382 tumor specimens from PC patients were examined for candidate m5C-related gene and protein expression by using quantitative PCR (qPCR) and immunohistochemistry (IHC). The proliferation rate of PC cells was detected by MTS assay. Xen

SUBMITTER: Yang R 

PROVIDER: S-EPMC7804980 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature