Culturable bacteria associated with Anopheles darlingi and their paratransgenesis potential.

ABSTRACT:

Background

Malaria remains a major public health problem in South America, mostly in the Amazon region. Among newly proposed ways of controlling malaria transmission to humans, paratransgenesis is a promising alternative. Paratransgenesis aims to inhibit the development of parasites within the vector through the action of genetically modified bacteria. The first step towards successful paratransgenesis in the Amazon is the identification of Anopheles darlingi symbiotic bacteria, which are transmitted vertically among mosquitoes, and are not pathogenic to humans.

Methods

Culturable bacteria associated with An. darlingi and their breeding sites were isolated by conventional microbiological techniques. Isolated strains were transformed with a GFP expressing plasmid, pSPT-1-GFP,

SUBMITTER: Rocha EM 

PROVIDER: S-EPMC7805163 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature