Drug perturbation gene set enrichment analysis (dpGSEA): a new transcriptomic drug screening approach.

ABSTRACT:

Background

In this study, we demonstrate that our modified Gene Set Enrichment Analysis (GSEA) method, drug perturbation GSEA (dpGSEA), can detect phenotypically relevant drug targets through a unique transcriptomic enrichment that emphasizes biological directionality of drug-derived gene sets.

Results

We detail our dpGSEA method and show its effectiveness in detecting specific perturbation of drugs in independent public datasets by confirming fluvastatin, paclitaxel, and rosiglitazone perturbation in gastroenteropancreatic neuroendocrine tumor cells. In drug discovery experiments, we found that dpGSEA was able to detect phenotypically relevant drug targets in previously published differentially expressed genes of CD4+T regulatory cells from immune responders and non-respond

SUBMITTER: Fang M 

PROVIDER: S-EPMC7805197 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature